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Journal: STAR Protocols

Article Title: Protocol for enhancing Cas9 efficiency and fidelity through structure-guided phosphate-locking loop engineering

doi: 10.1016/j.xpro.2026.104452

Figure Lengend Snippet:

Article Snippet: 2× Phanta Max Master Mix (Dye Plus) , Vazyme , Cat# P525-01.

Techniques: Virus, Recombinant, Saline, Plasmid Preparation, Cell Culture, Gel Extraction, Purification, Multiplex Assay, Transfection, Mutagenesis, Amplification, Sequencing, Expressing, Variant Assay, Control, Software, Drug discovery, Biomarker Discovery, Spectrophotometry, Hood, Electrophoresis, Imaging